Rapid Allergen Tests - Jargon Buster

Allergen Management is a key talking point throughout the food production industry, with sites working harder than ever to ensure consumer safety and secure audit compliance.

With this in mind, we have put together a guide to the key terms surrounding one of the most popular allergen testing methods: Rapid Allergen Tests (Lateral Flow Immunoassays).

As a leading supplier of these tests, we are perfectly positioned to support you with any queries relating to this topic. So, if you can’t find the term you are looking for in the list below or need any other form of help with your site’s allergen management strategy, contact us on
01473 461800 or sales@klipspringer.com. 


Lateral Flow Immunoassays (LFIs) 

A quick, easy, and cost-effective testing method that can be used to detect the presence of a target allergen (e.g. gluten) in a liquid sample. Due to their specificity, LFIs are unable to assess several allergens simultaneously.  

Sample Application Slot 

This is the window on the LFI test strip where you drop 200μL (the equivalent of four drops) of your test solution or rinse water.  

Confirmation Window 

This is the window on the LFI test strip that conveys the validity of your results. Once your sample has been applied to the Sample Application Slot, the Confirmation Window should change from clear to a red-purple. If the window remains clear, the test is invalid, and you will need to retest the sample with a new stick.  

Test Window 

This is the window on the LFI test strip where your results show up. If a red-purple line appears, the test result is positive. If the window remains clear, but the Confirmation Window turns a red-purple, the test result is negative. 

High Dose Hook Effect 

If too much of a target allergen is present in a sample, the strip inside the LFI will become overloaded and produce a negative test result. This is known as the High Dose Hook Effect.

Click here for more information and to discover the solution. 

Limit of Detection / Detection Accuracy  

This relates to the accuracy of your test results and may vary depending on the allergen being tested for.  

Klipspringer’s Range of Rapid Allergen EASY LFIs have a detection accuracy of 0.5ppm for rinse water and 4/5ppm for surfaces.

Parts Per Million (ppm) 

Parts Per Million (ppm) is a unit that expresses the concentration of a substance in a solution. In this instance, the ppm is a ratio of the allergen in milligrams per kilogram of the sample being tested.  

Assay Time 

This is the time it takes for your test to generate a result. It doesn’t include the time it takes to set up your equipment, prepare your sample ect…  

Klipspringer’s Range of Rapid Allergen Tests have an assay time of 10 minutes.  

Extraction Buffer Solution 

Some LFIs require you to prepare your sample using an Extraction Buffer Solution. This is a solution that breaks open cells and stabilises target compounds ready for testing.  

Klipspringer’s Range of Rapid Allergen LFIs do not require an Extraction Buffer Solution, making the testing process quicker and easier for your operatives.  

Cleaning Validation 

This is the process of proving the cleaning methods performed at your factory are effective in removing allergens and reducing the risk of cross contamination. Depending on the applications and risk levels at your site, this could be carried out with the help of Lateral Flow Immunoassays. 

Cleaning Verification 

This is the process of verifying your cleaning program. It will typically occur on a daily basis, so it is important to find a quick, easy-to-use, and low-cost approach that is still incredibly accurate. The most popular options for daily verification are visual inspection, ATP tests, protein-specific swabs, and the A3 system.Â